Plasmid

Part:BBa_K4687006:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


pEC-XK99E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 92
    Illegal NheI site found at 850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1382
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 2046
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1895
    Illegal SapI.rc site found at 2105
    Illegal SapI.rc site found at 3078


Design Notes

Plasmid vectors introduced the CRISPR components into the cells to ensure that they function properly within the cell. By selecting different plasmid vectors for experiments, the most suitable ones were selected to improve the efficiency of gene editing.


Source

E.coli-Corynebacterium glutamicum shuttle expression vectors based on the medium-copy-no plasmid pGA1 and containing the Ptrc promoter.

References